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KMID : 0378019890320010059
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New Medical Journal
1989 Volume.32 No. 1 p.59 ~ p.68
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Effect of Dimethyl Sulfoxide on the Aculte Ischemic Bowel in Rats
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Abstract
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This study aimed at investigating the effect and the action mechanism of Dimethyl Salfoxide (DM O) on the acute intestinal ischemia of the rats. Acute intestinal ischemia was induced by the segmental ligation of ileum, and then reperfused after 30 minutes, l hour, 3 hours and 5 hours, respectively. Immedi tely after reperfusion, experimental group received 5 ml of 20% DMSO into the right femoral vein at a constant rate (0.5ml/min.) using an infusion pump, and the control group received physiologic saline at the s. me rate and volume. On 24 hours after operation, gross and hitologic (H & E, and epona-toluidine blue St. in) examination were performed.
In another series of experiments, the temperature difference between the ischemic and normal bowel was recorded with use of a sensitive electronic thermometer at various time interval (0, 10, 20, 30, 40 min. and 24 hours) after reperfusion.
The results were as follows
1. Gross examination
The observed macroscopic lesions in the DMSO treated group of 30 min. or 1 hour ischemia w-re significantly milder than those of control group. However, there was no significant difference betty, en the control and DMSO treatment groups in rats of 3 hours of 5 hours ischemia.
2. Histologic examination
The control group of 1 hour ischemia showed epithelial necrosis, intense infiltration of inflammatory cells, disintegration of lamia propria and focal degeneration of muscle layer. In the DMSO treated gro p, however, the histologic changes were far milder than those of control group. In the segment ischemic i or 3 hours or 5 hours, extensive transmural necrosis was noticed in both control and DMSO treated groups.
3. Temperature change of ischemic bowel
In the segment ischemic for 30 min., the recovery rate of temperature after reperfusion in the DM .O treated group was slightly faster than that of control group, but not significant. In the segment ische is for 1 hour, there was no recovery of of temperature until 40 min. after reperfusion in both groups, but the temperature of the DMSO treated group was recovered to normal range in 24 hours after reperfusion. The temperature of the segment ischemic for 3 hours and 5 hours was maintained much lower degree
than that of normal bowel until 24 hours after reperfusion.
These experimental results demonstrated that DMSO has a significant protective effect on the acute intestinal ischemia, and the actual mechanism of the effect on the acute intestinal ischemia, and the actual
mechanism of the effect seem to exhibit through combination of complex mechanism including vasodilation and free radical scavenging action.
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